Western blot (WB): | 1:2000 |
Immunofluorescence (IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-JUN antibody, and then analyzed by flow cytometry.
Figure 2. Flow cytometric Analysis of Hela cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 3. Anti-JUN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN .
Figure 4. Flow cytometric Analysis of Jurkat cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.
Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-JUN antibody, and then analyzed by flow cytometry.
Figure 2. Flow cytometric Analysis of Hela cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 3. Anti-JUN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN .
Figure 4. Flow cytometric Analysis of Jurkat cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.