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Anti-c-Jun/JUN Antibody (Clone#OTI3G2)

Mouse monoclonal antibody

说明书

M02038-2

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-JUN antibody, and then analyzed by flow cytometry.

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  • Figure 2. Flow cytometric Analysis of Hela cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  • Figure 3. Anti-JUN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN .

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  • Figure 4. Flow cytometric Analysis of Jurkat cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  • Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.

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产品名称
Anti-c-Jun/JUN Antibody (Clone#OTI3G2)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Activator protein 1; AP 1; AP1; c Jun; JUN; jun oncogene; P39; Proto oncogene c Jun; Transcription factor AP 1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IF, FCM
基因名称
JUN
克隆号
OTI3G2
抗体来源
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human JUN (NP_002219) produced in HEK293T cell.
计算分子量
163 kDa
实际分子量
35.5 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]
Uniprot ID
P05412  
文献引用格式
c-Jun/JUN Antibody (Clone#OTI3G2) (Boster Biological Technology, Wuhan, China. Catalog#M02038-2)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
Jun proto-oncogene, AP-1 transcription factor subunit
蛋白名全称
Transcription factor AP-1
推荐稀释比
Western blot (WB):1:2000
Immunofluorescence (IF):1:100
Flow cytometry (FCM):1:100

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