Western blot (WB): | 1:500-2000 |
ImmunoPrecipitation (IP): | 1:20 |
Western blot analysis of anti-P-JUN antibody (BM4717). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-JUN antigen affinity purified monoclonal antibody (BM4717) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-JUN at approximately 45 kDa. The expected band size for P-JUN is at 36 kDa.
Western blot analysis of anti-P-JUN antibody (BM4717). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-JUN antigen affinity purified monoclonal antibody (BM4717) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-JUN at approximately 45 kDa. The expected band size for P-JUN is at 36 kDa.