Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human MDA-MB-231 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human MDA-MB-231 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human MDA-MB-231 whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
Western blot analysis of CD1B using anti-CD1B antibody (A02158-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD1B using anti-CD1B antibody (A02158-1).
CD1B was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD1B Antibody (A02158-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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