Anti-CD1B Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A02158-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD1B using anti-CD1B antibody (A02158-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HCCT whole cell lysates,
Lane 2: MOLT4 whole cell lysates,
Lane 3: Junkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (A02158-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
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产品简介>

产品名称

Anti-CD1B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD1b molecule; CD1; CD1A; R1; CD1B

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

CD1B

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD1B recombinant protein (Position: D78-Y110).

计算分子量

37 kDa

实际分子量

40 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.

Uniprot ID

P29016

文献引用格式

CD1B Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02158-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD1b molecule

蛋白名全称

T-cell surface glycoprotein CD1b

实验方案>

实验步骤

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