Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody (A04372-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2).
ACSL4/FACL4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing HepG2 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 6. Flow Cytometry analysis of U87 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing U87 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody (A04372-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2).
ACSL4/FACL4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing HepG2 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 6. Flow Cytometry analysis of U87 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing U87 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACSL4/FACL4 antigen affinity purified polyclonal antibody (A04372-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2) .
ACSL4/FACL4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2).
ACSL4/FACL4 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing HepG2 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 6. Flow Cytometry analysis of U87 cells using anti-ACSL4/FACL4 antibody (A04372-2).
Overlay histogram showing U87 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL4/FACL4 Antibody (A04372-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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