Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of anti-ACSL4 Antibody (A04372-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: CACO-2 whole cell lysates,
Lane 5: HEL whole cell lysates,
Lane 6: U-87MG whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat lung tissue lysates,
Lane 9: rat stomach tissue lysates,
Lane 10: PC-12 whole cell lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse lung tissue lysates,
Lane 13: mouse stomach tissue lysates,
Lane 14: NIH/3T3 whole cell lysates.
Use rabbit anti-ACSL4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACSL4 at approximately 79KD. The expected band size for ACSL4 is at 79KD.
Figure 1. Western blot analysis of anti-ACSL4 Antibody (A04372-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: CACO-2 whole cell lysates,
Lane 5: HEL whole cell lysates,
Lane 6: U-87MG whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat lung tissue lysates,
Lane 9: rat stomach tissue lysates,
Lane 10: PC-12 whole cell lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse lung tissue lysates,
Lane 13: mouse stomach tissue lysates,
Lane 14: NIH/3T3 whole cell lysates.
Use rabbit anti-ACSL4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACSL4 at approximately 79KD. The expected band size for ACSL4 is at 79KD.