Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACSL4/FACL4 antigen affinity purified monoclonal antibody (M04372) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Rectal moderately differentiatedadenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (M04372).
Overlay histogram showing HepG2 cells stained with M04372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACSL4/FACL4 antigen affinity purified monoclonal antibody (M04372) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Rectal moderately differentiatedadenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (M04372).
Overlay histogram showing HepG2 cells stained with M04372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACSL4/FACL4 antigen affinity purified monoclonal antibody (M04372) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.
Figure 2. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in a paraffin-embedded section of human Rectal moderately differentiatedadenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
ACSL4/FACL4 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (M04372).
Overlay histogram showing HepG2 cells stained with M04372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
联系我们800-880-8748
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390 8008808748 技术支持：武汉丰网
© 1993-2024 Boster Biological Technology Co.,Ltd E-mail:boster@boster.com 鄂ICP备05005548号-2 
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
成功添加到购物车
微信客服
微信扫一扫立即咨询 