Figure 1. Western blot analysis of anti- HSP90AA1 antibody (BA0369). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates,
Lane 8: human Hela whole cell lysates,
Lane 9: rat lung tissue lysates,
Lane 10: mouse Ana-1 whole cell lysates.
Use rabbit anti- HSP90AA1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for HSP90AA1 at approximately 90KD. The expected band size for HSP90AA1 is at 85KD.
Figure 2. IHC analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 4. Flow cytometry analysis of SiHa cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- HSP90AA1 antibody (BA0369). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates,
Lane 8: human Hela whole cell lysates,
Lane 9: rat lung tissue lysates,
Lane 10: mouse Ana-1 whole cell lysates.
Use rabbit anti- HSP90AA1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for HSP90AA1 at approximately 90KD. The expected band size for HSP90AA1 is at 85KD.
Figure 2. IHC analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 4. Flow cytometry analysis of SiHa cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- HSP90AA1 antibody (BA0369). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates,
Lane 8: human Hela whole cell lysates,
Lane 9: rat lung tissue lysates,
Lane 10: mouse Ana-1 whole cell lysates.
Use rabbit anti- HSP90AA1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for HSP90AA1 at approximately 90KD. The expected band size for HSP90AA1 is at 85KD.
Figure 2. IHC analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IF analysis of Hsp90 alpha/HSP90AA1 using anti-Hsp90 alpha/HSP90AA1 antibody (BA0369).
Hsp90 alpha/HSP90AA1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 Antibody (BA0369) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 4. Flow cytometry analysis of SiHa cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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