Western blot (WB): | 1:2000 |
Figure 1. Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-HSP90AA1 monoclonal antibody (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSP90AA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSP90AA1(Cat# M01103-5).
Figure 1. Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-HSP90AA1 monoclonal antibody (1:200).
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSP90AA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSP90AA1(Cat# M01103-5).