Anti-Hsp90 alpha/HSP90AA1 Antibody (Clone#BAC-8)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP

BM4067

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-Hsp90 alpha/HSP90AA1 antibody (BM4067). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse HEPA1-6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Hsp90 alpha/HSP90AA1 antigen affinity purified monoclonal antibody (BM4067) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Hsp90 alpha/HSP90AA1 at approximately 85 kDa. The expected band size for Hsp90 alpha/HSP90AA1 is at 85 kDa.
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Immunofluorescent analysis of Hela cells, using Hsp90 alpha Antibody .
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产品名称

Anti-Hsp90 alpha/HSP90AA1 Antibody (Clone#BAC-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Heat shock 86 kDa; HSP 86; HSP86; Hsp89; HSP89A; HSP90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP2

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, ICC/IF, IP

基因名称

HSP90AA1

克隆号

BAC-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Hsp90 alpha

计算分子量

85 kDa

实际分子量

85 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90-kDa heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.

Uniprot ID

P07900

文献引用格式

Hsp90 alpha/HSP90AA1 Antibody (Clone#BAC-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4067)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Heat shock protein HSP 90-alpha

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实验步骤

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