Anti-IL6 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

BA0562-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (BA0562-2).
Lane 1: recombinant human IL-6 protein 10ng,
Lane 2: recombinant human IL-6 protein 5ng,
Lane 3: recombinant human IL-6 protein 2.5ng .
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (BA0562-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL6 at approximately 24 kDa.
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产品名称

Anti-IL6 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

IL6

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human IL-6.

计算分子量

24 kDa

实际分子量

24 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL6 gene to chromosome 7p15.

Uniprot ID

P05231

文献引用格式

IL6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0562-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 6(interferon, beta 2)

蛋白名全称

Interleukin-6(IL-6)

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实验步骤

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