Anti-IL6 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ELISA(Cap)

A00102-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (A00102-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: Ana-1 whole cell lysates,
Lane 4: RAW264.7 whole cell lysates,
Lane 5: EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (A00102-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
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产品名称

Anti-IL6 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, ELISA(Cap)

基因名称

Il6

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, which shares 87.5% amino acid (aa) sequence identity with mouse IL6.

计算分子量

24 kDa

实际分子量

30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, the IL6 gene is mapped to chromosome 7p15.

Uniprot ID

P20607

RRID

AB_3080949

文献引用格式

IL6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00102-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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