Anti-IL6 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA4339

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IL6 using anti-IL6 antibody (BA4339). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse smooth muscle tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse Hepa1/6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse SP/20 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (BA4339) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
all(3)
Figure 2. Western blot analysis of IL6 using anti-IL6 antibody (BA4339). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat smooth muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse small intestine tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: rat RH-35 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (BA4339) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.
all(3)
Figure 3. IHC analysis of IL6 using anti-IL6 antibody (BA4339) .
IL6 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-IL6 Antibody (BA4339) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IL6 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

IL6

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, different from the related mouse sequence by two amino acids.

计算分子量

24 kDa

实际分子量

30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL6 gene to chromosome 7p15.

Uniprot ID

P20607

文献引用格式

IL6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4339)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 6(interferon, beta 2)

蛋白名全称

Interleukin-6(IL-6)

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实验步骤

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