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Anti-ABCC1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0567

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of ABCC1 using anti-ABCC1 antibody (BA0567). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: JURKAT whole cell lysates,
    Lane 2: CEM whole cell lysates,
    Lane 3: A549 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCC1 antigen affinity purified polyclonal antibody (BA0567) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCC1 at approximately 220 kDa. The expected band size for ABCC1 is at 172 kDa.

    all(2)
  • IHC analysis of ABCC1 using anti-ABCC1 antibody (BA0567).
    ABCC1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ABCC1 Antibody (BA0567) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-ABCC1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ABC29; ABCC; ABCC1; Leukotriene C(4) transporter; LTC4 transporter; MRP; MRP1
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
ABCC1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human MRP1, different from the related rat and mouse sequences by one amino acid.
计算分子量
172 kDa
实际分子量
220 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
Uniprot ID
P33527  
文献引用格式
ABCC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0567)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
ATP-binding cassette, sub-family C(CFTR/MRP), member 1
蛋白名全称
Multidrug resistance-associated protein 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-ABCC1 Antibody

    筛选器: All WB IHC

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