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Anti-Beta Amyloid/APP Antibody

Rabbit polyclonal antibody

说明书

筛选器: All WB IHC

BA0581

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of Beta Amyloid/APP using anti-Beta Amyloid/APP antibody (BA0581). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Amyloid/APP antigen affinity purified polyclonal antibody (BA0581) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Amyloid/APP at approximately 87-120 kDa. The expected band size for Beta Amyloid/APP is at 87 kDa.

    all(2)
  • Figure 2. IHC analysis of Beta Amyloid/APP using anti-Beta Amyloid/APP antibody (BA0581).
    Beta Amyloid/APP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid/APP Antibody (BA0581) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

    all(2)

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产品简介>

产品名称
Anti-Beta Amyloid/APP Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ABETA; ABPP; amyloid beta 1-42; Amyloid beta A4 protein; amyloid P; amyloid precursor protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; APP; APPI; Aβ1-42; beta Amyloid
产品类型
Polyclonal
检验物种
human, mouse, rabbit, rat
应用范围
WB, IHC
基因名称
APP
抗体来源
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human APP identical to the related mouse and rat sequences.
计算分子量
87 kDa
实际分子量
87-120 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Amyloid precursor protein(APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid(Abeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.
Uniprot ID
P05067  
文献引用格式
Beta Amyloid/APP Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0581)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
amyloid beta(A4) precursor protein
蛋白名全称
Amyloid beta A4 protein
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Beta Amyloid/APP Antibody

    筛选器: All WB IHC

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