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Anti-Beta Amyloid/APP Antibody (Clone#OTI7G9)

Mouse monoclonal antibody

说明书

MA00081

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00081, Dilution 1:50)

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  • Figure 2. HEK293T cells transfected with either pCMV6-ENTRY APP (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (MA00081), and then analyzed by flow cytometry.

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  • Figure 3. Immunofluorescent staining of HepG2 cells using anti-APP mouse monoclonal antibody.

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  • Figure 4. Anti-APP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP .

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  • Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP (MA00081).

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产品简介>

产品名称
Anti-Beta Amyloid/APP Antibody (Clone#OTI7G9)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ABETA; ABPP; amyloid beta 1-42; Amyloid beta A4 protein; amyloid P; amyloid precursor protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; APP; APPI; Aβ1-42; beta Amyloid
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
APP
克隆号
OTI7G9
抗体来源
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human APP (NP_000475) produced in HEK293T cell.
实际分子量
87.0 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. It is mapped to 21q21.3. This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Uniprot ID
P05067   
文献引用格式
Beta Amyloid/APP Antibody (Clone#OTI7G9) (Boster Biological Technology, Wuhan, China. Catalog#MA00081)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
amyloid beta (A4) precursor protein
蛋白名全称
Amyloid beta A4 protein
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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