Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:50 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
Figure 1. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00081, Dilution 1:50)
Figure 2. HEK293T cells transfected with either pCMV6-ENTRY APP (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (MA00081), and then analyzed by flow cytometry.
Figure 3. Immunofluorescent staining of HepG2 cells using anti-APP mouse monoclonal antibody.
Figure 4. Anti-APP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP .
Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP (MA00081).
Figure 1. Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-APP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00081, Dilution 1:50)
Figure 2. HEK293T cells transfected with either pCMV6-ENTRY APP (Red) or empty vector control plasmid (Blue) were immunostained with anti-APP mouse monoclonal (MA00081), and then analyzed by flow cytometry.
Figure 3. Immunofluorescent staining of HepG2 cells using anti-APP mouse monoclonal antibody.
Figure 4. Anti-APP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY APP .
Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP (MA00081).