Figure 1. Western blot analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Amyloid protein 42/APP antigen affinity purified polyclonal antibody (PB9091) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Amyloid protein 42/APP at approximately 87-120 kDa. The expected band size for Beta Amyloid protein 42/APP is at 87 kDa.
Figure 2. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of APP using anti- APP antibody (PB9091).
APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- APP Antibody (PB9091) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Amyloid protein 42/APP antigen affinity purified polyclonal antibody (PB9091) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Amyloid protein 42/APP at approximately 87-120 kDa. The expected band size for Beta Amyloid protein 42/APP is at 87 kDa.
Figure 2. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of APP using anti- APP antibody (PB9091).
APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- APP Antibody (PB9091) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 1. Western blot analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Amyloid protein 42/APP antigen affinity purified polyclonal antibody (PB9091) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Amyloid protein 42/APP at approximately 87-120 kDa. The expected band size for Beta Amyloid protein 42/APP is at 87 kDa.
Figure 2. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of Beta Amyloid protein 42/APP using anti-Beta Amyloid protein 42/APP antibody (PB9091).
Beta Amyloid protein 42/APP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Beta Amyloid protein 42/APP Antibody (PB9091) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of APP using anti- APP antibody (PB9091).
APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- APP Antibody (PB9091) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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