Anti-ATM Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

BA0655-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of ATM using anti-ATM antibody (BA0655-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates ,
Lane 2: U87 whole cell lysates ,
Lane 3: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (BA0655-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATM at approximately 350 kDa. The expected band size for ATM is at 351 kDa.
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Figure 2. Western blot analysis of ATM using anti-ATM antibody (BA0655-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates ,
Lane 2: SMMC whole cell lysates ,
Lane 3: U87 whole cell lysates ,
Lane 4: A549 whole cell lysates ,
Lane 5: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (BA0655-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATM at approximately 350 kDa. The expected band size for ATM is at 351 kDa.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATM Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

A T mutated; AT1; ATA; ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; ATM; Serine protein kinase ATM; TEL1; TELO1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ATM

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human ATM, different from the related rat and mouse sequences by two amino acids.

计算分子量

351 kDa

实际分子量

350 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATM(ataxia telangiectasia mutated),also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. Linkage analysis of ataxia-telangiectasia led to mapping of the ATM gene to chromosome 11q22.3. Using an antiserum developed to a peptide corresponding to the deduced amino acid sequence of ATM, the ATM protein is a single, high molecular weight protein predominantly confined to the nucleus of human fibroblasts, although it is present in both nuclear and microsomal fractions from human lymphoblast cells and peripheral blood lymphocytes. Overexpression of ATM cDNA in AT cells enhanced their survival after radiation exposure, decreased radiation-induced chromosome aberrations, reduced radioresistant DNA synthesis, and partially corrected defective cell cycle checkpoints and induction of stress-activated protein kinase. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors, in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Uniprot ID

Q13315

文献引用格式

ATM Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0655-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ataxia telangiectasia mutated

蛋白名全称

Serine-protein kinase ATM

实验方案>

实验步骤

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