Anti-ATM Antibody (Clone#BCF-1)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF FCM

BM4091

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期： 5-7天

加入购物车立即购买

Figure 1. Western blot analysis of anti-ATM antibody (BM4091). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATM antigen affinity purified monoclonal antibody (BM4091) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATM at approximately 351 kDa. The expected band size for ATM is at 351 kDa.
all(4)
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ATM Antibody.
all(4)
Immunofluorescent analysis of Hela cells, using ATM Antibody.
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATM Antibody (Clone#BCF-1)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

A T mutated; AT1; ATA; ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; ATM; Serine protein kinase ATM; TEL1; TELO1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ATM

克隆号

BCF-1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ATM

计算分子量

351 kDa

实际分子量

350 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATM(ataxia telangiectasia mutated), also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. The ATM gene is mapped to chromosome 11q22.3. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Uniprot ID

Q13315

文献引用格式

ATM Antibody (Clone#BCF-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4091)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

实验步骤

引用文献(0)>

清除