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Anti-PKC Eta/PRKCH Antibody

Rabbit polyclonal antibody

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筛选器: All IHC WB

BA1361

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • IHC analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (BA1361).
    PKC Eta/PRKCH was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Eta/PRKCH Antibody (BA1361) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(4)
  • IHC analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (BA1361).
    PKC Eta/PRKCH was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Eta/PRKCH Antibody (BA1361) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(4)
  • IHC analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (BA1361).
    PKC Eta/PRKCH was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Eta/PRKCH Antibody (BA1361) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(4)

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产品名称
Anti-PKC Eta/PRKCH Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
protein kinase C eta; PKC-L; PKCL; PRKCL; nPKC-eta; PRKCH
产品类型
Polyclonal
检验物种
human, mouse, rat, rabbit
应用范围
IHC, WB
基因名称
PRKCH
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.
计算分子量
78 kDa
实际分子量
78 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
Uniprot ID
P24723  
文献引用格式
PKC Eta/PRKCH Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1361)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
protein kinase C, eta
蛋白名全称
Protein kinase C eta type
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-PKC Eta/PRKCH Antibody

    筛选器: All IHC WB

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