Anti-PKC Eta/PRKCH Antibody (Clone#ADAH-16)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM5367

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-PKC Eta/PRKCH antibody (BM5367). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Eta/PRKCH antigen affinity purified monoclonal antibody (BM5367) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Eta/PRKCH at approximately 78 kDa. The expected band size for PKC Eta/PRKCH is at 78 kDa.
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IHC analysis of PRKCH using anti-PRKCH antibody (BM5367) .
PRKCH was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-PRKCH Antibody (BM5367) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of PRKCH using anti-PRKCH antibody (BM5367) .
PRKCH was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-PRKCH Antibody (BM5367) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-PKC Eta/PRKCH Antibody (Clone#ADAH-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

protein kinase C eta; PKC-L; PKCL; PRKCL; nPKC-eta; PRKCH

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

PRKCH

克隆号

ADAH-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PKC eta PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

计算分子量

78 kDa

实际分子量

78 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Uniprot ID

P24723

文献引用格式

PKC Eta/PRKCH Antibody (Clone#ADAH-16) (Boster Biological Technology, Wuhan, China. Catalog#BM5367)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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