Western blot analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (PB9556). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: SKOV whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Eta/PRKCH antigen affinity purified polyclonal antibody (PB9556) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Eta/PRKCH at approximately 78 kDa. The expected band size for PKC Eta/PRKCH is at 78 kDa.
Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing A431 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(5) 
Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing K562 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(5) 
Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing MCF-7 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(5) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 23 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (PB9556). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: SKOV whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Eta/PRKCH antigen affinity purified polyclonal antibody (PB9556) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Eta/PRKCH at approximately 78 kDa. The expected band size for PKC Eta/PRKCH is at 78 kDa.
Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing A431 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing K562 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing MCF-7 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (PB9556). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: SKOV whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Eta/PRKCH antigen affinity purified polyclonal antibody (PB9556) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Eta/PRKCH at approximately 78 kDa. The expected band size for PKC Eta/PRKCH is at 78 kDa.
Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing A431 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing K562 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody (PB9556).Overlay histogram showing MCF-7 cells stained with PB9556 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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