Anti-APEX1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF

BA2105

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-APEX1 antibody (BA2105). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Hepa1-6 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates,
Lane 9: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (BA2105) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.
all(9)
IHC analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of human prostatic cancer tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IF analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:100. DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150)was used as secondary antibody.
all(9)
IF analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:100. DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150)was used as secondary antibody.
all(9)
IF analysis of APEX1 using anti-APEX1 antibody (BA2105).
APEX1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-APEX1 Antibody (BA2105) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-APEX1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

AP endonuclease 1; APE; APE1; APE1; APEX1; APEN; APEX; APEX nuclease; APEX1; APX; HAP1; Protein REF 1; REF1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF

基因名称

APEX1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human APE1, identical to the related rat and mouse sequences.

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Uniprot ID

P27695

文献引用格式

APEX1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2105)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

APEX nuclease(multifunctional DNA repair enzyme) 1

蛋白名全称

DNA-(apurinic or apyrimidinic site) lyase

实验方案>

实验步骤

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