Anti-APEX1 Antibody (Clone#5C11)

筛选器： All WB IHC FCM

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  Western blot analysis of anti-APEX1 antibody (M00627). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human COLO-320 whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: Rabbit IgG,
  Lane 6: Marker 1113,
  Lane 7: human A549 whole cell lysates,
  Lane 8: human PANC-1 whole cell lysates,
  Lane 9: human 22RV1 whole cell lysates,
  Lane 10: human MDA-MB-453 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-APEX1 antigen affinity purified monoclonal antibody (M00627) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.

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  IHC analysis of APEX1 using anti-APEX1 antibody (M00627).
  APEX1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with mouse anti-APEX1 Antibody (M00627) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of SiHa cells using anti-APEX1 antibody (M00627).
  Overlay histogram showing SiHa cells stained with M00627 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APEX1 Antibody (M00627) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti-APEX1 antibody (M00627). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human COLO-320 whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: Rabbit IgG,
  Lane 6: Marker 1113,
  Lane 7: human A549 whole cell lysates,
  Lane 8: human PANC-1 whole cell lysates,
  Lane 9: human 22RV1 whole cell lysates,
  Lane 10: human MDA-MB-453 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-APEX1 antigen affinity purified monoclonal antibody (M00627) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.

• 

  IHC analysis of APEX1 using anti-APEX1 antibody (M00627).
  APEX1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with mouse anti-APEX1 Antibody (M00627) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of SiHa cells using anti-APEX1 antibody (M00627).
  Overlay histogram showing SiHa cells stained with M00627 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APEX1 Antibody (M00627) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.