Anti-APEX1 Antibody (Clone#17A44)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM5218

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-APEX1 antibody (BM5218). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APEX1 antigen affinity purified monoclonal antibody (BM5218) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.
all(5)
IHC analysis of APEX1 using anti-APEX1 antibody (BM5218) .
APEX1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BM5218) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of APEX1 using anti-APEX1 antibody (BM5218) .
APEX1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BM5218) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of APEX1 using anti-APEX1 antibody (BM5218) .
APEX1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BM5218) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of APEX1 using anti-APEX1 antibody (BM5218) .
APEX1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-APEX1 Antibody (BM5218) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-APEX1 Antibody (Clone#17A44)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AP endonuclease 1; APE; APE1; APE1; APEX1; APEN; APEX; APEX nuclease; APEX1; APX; HAP1; Protein REF 1; REF1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

APEX1

克隆号

17A44

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human APE1

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway. Repair activities of Ape1 are stimulated by interaction with XRCC1, another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation.

Uniprot ID

P27695

文献引用格式

APEX1 Antibody (Clone#17A44) (Boster Biological Technology, Wuhan, China. Catalog#BM5218)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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