Anti-HSC70/HSPA8 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF FCM

BA2411

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (BA2411). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (BA2411) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
all(6)
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (BA2411).
HSC70/HSPA8 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (BA2411) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (BA2411).
HSC70/HSPA8 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (BA2411) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (BA2411).
HSC70/HSPA8 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (BA2411) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
ICC/IF analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (BA2411).
HSC70/HSPA8 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HSC70/HSPA8 Antibody (BA2411) at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
Flow Cytometry analysis of HL-60 cells using anti-HSC70/HSPA8 antibody (BA2411).
Overlay histogram showing HL-60 cells stained with BA2411 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSC70/HSPA8 Antibody (BA2411) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HSC70/HSPA8 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; HSPA8

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

HSPA8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsc70, different from the related mouse and rat sequences by one amino acid.

计算分子量

71 kDa

实际分子量

71 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HSPA8(heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. The HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly(A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.

Uniprot ID

P11142

文献引用格式

HSC70/HSPA8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2411)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock 70kDa protein 8

蛋白名全称

Heat shock cognate 71 kDa protein

实验方案>

实验步骤

引用文献(0)>

清除