Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Rat spleen tissue lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Rat kidney tissue lysates,
Lane 5: Mouse liver tissue lysates,
Lane 6: Mouse spleen tissue lysates,
Lane 7: Mouse brain tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: K562 whole cell lysates,
Lane 7: PC-3 whole cell lysates,
Lane 8: U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HSPA8 using anti-HSPA8 antibody (PB0678).HSPA8 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA8 Antibody (PB0678) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(8) 
IP analysis of HSPA8/HSC70 using anti-HSPA8/HSC70 antibody (PB0678) in A549 whole cell lysate.
Western blot analysis of HSPA8/HSC70 using anti- HSPA8/HSC70 antibody (PB0678).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- HSPA8/HSC70 antibody in A549 whole cell lysate,
Lane 3: anti- HSPA8/HSC70 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HSPA8/HSC70 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSPA8/HSC70 at approximately 71 kDa. The expected band size for HSPA8/HSC70 is at 71 kDa.
Flow Cytometry analysis of K562 cells using anti-HSC70/HSPA8 antibody (PB0678).
Overlay histogram showing K562 cells stained with PB0678 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:250-300 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Rat spleen tissue lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Rat kidney tissue lysates,
Lane 5: Mouse liver tissue lysates,
Lane 6: Mouse spleen tissue lysates,
Lane 7: Mouse brain tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: K562 whole cell lysates,
Lane 7: PC-3 whole cell lysates,
Lane 8: U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HSPA8 using anti-HSPA8 antibody (PB0678).HSPA8 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA8 Antibody (PB0678) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IP analysis of HSPA8/HSC70 using anti-HSPA8/HSC70 antibody (PB0678) in A549 whole cell lysate.
Western blot analysis of HSPA8/HSC70 using anti- HSPA8/HSC70 antibody (PB0678).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- HSPA8/HSC70 antibody in A549 whole cell lysate,
Lane 3: anti- HSPA8/HSC70 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HSPA8/HSC70 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSPA8/HSC70 at approximately 71 kDa. The expected band size for HSPA8/HSC70 is at 71 kDa.
Flow Cytometry analysis of K562 cells using anti-HSC70/HSPA8 antibody (PB0678).
Overlay histogram showing K562 cells stained with PB0678 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Rat spleen tissue lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Rat kidney tissue lysates,
Lane 5: Mouse liver tissue lysates,
Lane 6: Mouse spleen tissue lysates,
Lane 7: Mouse brain tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: K562 whole cell lysates,
Lane 7: PC-3 whole cell lysates,
Lane 8: U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PB0678).
HSC70/HSPA8 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HSPA8 using anti-HSPA8 antibody (PB0678).HSPA8 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA8 Antibody (PB0678) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IP analysis of HSPA8/HSC70 using anti-HSPA8/HSC70 antibody (PB0678) in A549 whole cell lysate.
Western blot analysis of HSPA8/HSC70 using anti- HSPA8/HSC70 antibody (PB0678).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- HSPA8/HSC70 antibody in A549 whole cell lysate,
Lane 3: anti- HSPA8/HSC70 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HSPA8/HSC70 antigen affinity purified polyclonal antibody (PB0678) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSPA8/HSC70 at approximately 71 kDa. The expected band size for HSPA8/HSC70 is at 71 kDa.
Flow Cytometry analysis of K562 cells using anti-HSC70/HSPA8 antibody (PB0678).
Overlay histogram showing K562 cells stained with PB0678 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HSC70/HSPA8 Antibody (PB0678) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
