Anti-HSC70/HSPA8 Antibody (Clone#OTI1H3)

Mouse monoclonal antibody

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M01024-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA8 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M01024-3)
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Anti-HSPA8 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA8(Cat# M01024-3).
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产品简介>

产品名称

Anti-HSC70/HSPA8 Antibody (Clone#OTI1H3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; HSPA8

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

HSPA8

克隆号

OTI1H3

宿主

Mouse

免疫原

Full length human recombinant protein of human HSPA8(NP_006588) produced in HEK293T cell.

计算分子量

71 kDa

实际分子量

71 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot ID

P11142

文献引用格式

HSC70/HSPA8 Antibody (Clone#OTI1H3) (Boster Biological Technology, Wuhan, China. Catalog#M01024-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock 70kDa protein 8

蛋白名全称

Heat shock cognate 71 kDa protein

实验方案>

实验步骤

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