Anti-5 Lipoxygenase/ALOX5 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA2703

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (BA2703). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SW620 whole cell lysates,
Lane 2: JURKAT whole cell lysates,
Lane 3: COLO320 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified polyclonal antibody (BA2703) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.
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Figure 2. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (BA2703).
5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (BA2703) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-5 Lipoxygenase/ALOX5 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

5 Lipoxygenase; 5 LO; 5 LOX; 5LPG; ALOX5; LOG5

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

ALOX5

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5, different from the related rat and mouse sequences by two amino acids.

计算分子量

78 kDa

实际分子量

78 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ALOX5(ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO(5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences(NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.

Uniprot ID

P09917

文献引用格式

5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2703)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 5-lipoxygenase

蛋白名全称

Arachidonate 5-lipoxygenase(5-LO/5-lipoxygenase)

实验方案>

实验步骤

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