Anti-5 Lipoxygenase/ALOX5 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All IHC WB

PB0008

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 2. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008).
5 Lipoxygenase/ALOX5 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-5 Lipoxygenase/ALOX5 Antibody (PB0008) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 5. Western blot analysis of 5 Lipoxygenase/ALOX5 using anti-5 Lipoxygenase/ALOX5 antibody (PB0008). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SGC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified polyclonal antibody (PB0008) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-5 Lipoxygenase/ALOX5 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

5 Lipoxygenase; 5 LO; 5 LOX; 5LPG; ALOX5; LOG5

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

ALOX5

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

计算分子量

78 kDa

实际分子量

78 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Uniprot ID

P09917

文献引用格式

5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0008)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 5-lipoxygenase

蛋白名全称

Arachidonate 5-lipoxygenase

实验方案>

实验步骤

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