Figure 1. Western blot analysis of anti-5 Lipoxygenase/ALOX5 antibody (BM5522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified monoclonal antibody (BM5522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| Flow Cytometry (FCM): | 1:50 |
Figure 1. Western blot analysis of anti-5 Lipoxygenase/ALOX5 antibody (BM5522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified monoclonal antibody (BM5522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.
Figure 1. Western blot analysis of anti-5 Lipoxygenase/ALOX5 antibody (BM5522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified monoclonal antibody (BM5522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.
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