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Anti-TFAM Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF IF

BA2827

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of TFAM using anti-TFAM antibody (BA2827). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: human Caco-2 whole cell lysates,
    Lane 4: human Raji whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAM antigen affinity purified polyclonal antibody (BA2827) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IHC analysis of TFAM using anti-TFAM antibody (BA2827) .
    TFAM was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(12)
  • IF analysis of TFAM using anti-TFAM antibody (BA2827).
    TFAM was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(12)
  • IF analysis of TFAM using anti-TFAM antibody (BA2827).
    TFAM was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(12)
  • IF analysis of TFAM using anti-TFAM antibody (BA2827).
    TFAM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (BA2827) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(12)

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产品简介>

产品名称
Anti-TFAM Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Cell and organelle markers; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker; MtTF1; mtTFA; TCF 6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6; Transcription factor 6 like 2
产品类型
Polyclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, IF
基因名称
TFAM
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA.
计算分子量
29 kDa
实际分子量
24 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
TFAM(Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al.(1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, or TCF6L3) are pseudogenes. Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
Uniprot ID
Q00059  
文献引用格式
TFAM Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2827)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
transcription factor A, mitochondrial
蛋白名全称
Transcription factor A, mitochondrial
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Immunofluorescence (IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-TFAM Antibody

    筛选器: All WB IHC ICC/IF IF

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