Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
ImmunoPrecipitation (IP): | 1:250-300 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of TFAM using anti-TFAM antibody (PB0413). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brian tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAM antigA03957-Aen affinity purified polyclonal antibody (PB0413) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.
IHC analysis of TFAM using anti-TFAM antibody (PB0413).
TFAM was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of TFAM using anti-TFAM antibody (PB0413).
TFAM was detected in a paraffin-embedded section of human pancrease cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of TFAM using anti-TFAM antibody (PB0413) and anti-Beta Tubulin antibody (M01857-3).
TFAM was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of TFAM using anti-TFAM antibody (PB0413) in K562 whole cell lysate.
Western blot analysis of TFAM using anti- TFAM antibody (PB0413).
Lane 1: K562 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- TFAM antibody in K562 whole cell lysate,
Lane 3: anti- TFAM antibody (2μg) + K562 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TFAM antigen affinity purified polyclonal antibody (PB0413) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.
Western blot analysis of TFAM using anti-TFAM antibody (PB0413). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brian tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAM antigA03957-Aen affinity purified polyclonal antibody (PB0413) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.
IHC analysis of TFAM using anti-TFAM antibody (PB0413).
TFAM was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of TFAM using anti-TFAM antibody (PB0413).
TFAM was detected in a paraffin-embedded section of human pancrease cancer tissue. The tissue section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of TFAM using anti-TFAM antibody (PB0413) and anti-Beta Tubulin antibody (M01857-3).
TFAM was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TFAM Antibody (PB0413) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of TFAM using anti-TFAM antibody (PB0413) in K562 whole cell lysate.
Western blot analysis of TFAM using anti- TFAM antibody (PB0413).
Lane 1: K562 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- TFAM antibody in K562 whole cell lysate,
Lane 3: anti- TFAM antibody (2μg) + K562 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TFAM antigen affinity purified polyclonal antibody (PB0413) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.