Anti-TFAM Antibody (Clone#4D9)

Mouse monoclonal antibody

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筛选器： All WB IHC FCM

M01119-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TFAM using anti-TFAM antibody (M01119-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 tissue lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-TFAM antigen affinity purified monoclonal antibody (M01119-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAM at approximately 24 kDa. The expected band size for TFAM is at 29 kDa.
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IHC analysis of TFAM using anti-TFAM antibody (M01119-1).
TFAM was detected in a paraffin-embedded section of human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TFAM Antibody (M01119-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of TFAM using anti-TFAM antibody (M01119-1).
TFAM was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TFAM Antibody (M01119-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Flow cytometry analysis of CACO-2 cell HELA(1:100) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).
all(4)

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产品名称

Anti-TFAM Antibody (Clone#4D9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker; MtTF1; mtTFA; TCF 6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6; Transcription factor 6 like 2

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

TFAM

克隆号

4D9

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA, different from the related mouse and rat sequences by five amino acids.

计算分子量

29 kDa

实际分子量

24 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

TFAM(Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al.(1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, or TCF6L3) are pseudogenes. Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Uniprot ID

Q00059

RRID

AB_3081823

文献引用格式

TFAM Antibody (Clone#4D9) (Boster Biological Technology, Wuhan, China. Catalog#M01119-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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