Anti-P62/SQSTM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BA2849

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P62/SQSTM1 antigen affinity purified polyclonal antibody (BA2849) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P62/SQSTM1 at approximately 62 kDa. The expected band size for P62/SQSTM1 is at 48 kDa.
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IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human Cholangiocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of human sarcoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(13)
IF analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BA2849).
P62/SQSTM1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-P62/SQSTM1 Antibody (BA2849) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(13)
Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(13)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-P62/SQSTM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SQSTM1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1, different from the related rat and mouse sequences by one amino acid.

计算分子量

48 kDa

实际分子量

62 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

SQSTM1(Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2(SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al.(1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al.(1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Uniprot ID

Q13501

文献引用格式

P62/SQSTM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2849)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

sequestosome 1

蛋白名全称

Sequestosome-1

实验方案>

实验步骤

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