Anti-P62/SQSTM1 Antibody (Clone#EBB-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4385

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P62/SQSTM1 antibody (BM4385). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P62/SQSTM1 antigen affinity purified monoclonal antibody (BM4385) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P62/SQSTM1 at approximately 62 kDa. The expected band size for P62/SQSTM1 is at 48 kDa.
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IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BM4385) .
P62/SQSTM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BM4385) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BM4385) .
P62/SQSTM1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BM4385) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (BM4385) .
P62/SQSTM1 was detected in a paraffin-embedded section of human squamous cell cervical carcinoma tissue. The tissue section was incubated with rabbit anti-P62/SQSTM1 Antibody (BM4385) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-P62/SQSTM1 Antibody (Clone#EBB-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SQSTM1

克隆号

EBB-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human p62/SQSTM1

计算分子量

48 kDa

实际分子量

62 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Uniprot ID

Q13501

文献引用格式

P62/SQSTM1 Antibody (Clone#EBB-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4385)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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