Anti-P62/SQSTM1 Antibody (Clone#OTI1E9)

Mouse monoclonal antibody

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筛选器： All WB FCM IHC ICC/IF

M00300-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-P62 antibody (M00300-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-P62 antigen affinity purified monoclonal antibody (M00300-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P62 at approximately 62 kDa. The expected band size for P62 is at 48 kDa.
all(6)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
all(6)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(6)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(6)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(6)
Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 .
all(6)

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产品简介>

产品名称

Anti-P62/SQSTM1 Antibody (Clone#OTI1E9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, FCM, IHC, ICC/IF

基因名称

SQSTM1

克隆号

OTI1E9

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.

计算分子量

48 kDa

实际分子量

62 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

Uniprot ID

Q13501

文献引用格式

P62/SQSTM1 Antibody (Clone#OTI1E9) (Boster Biological Technology, Wuhan, China. Catalog#M00300-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

sequestosome 1

蛋白名全称

Sequestosome-1

实验方案>

实验步骤

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