Anti-RIPK1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF

BA3852-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RIPK1 using anti-RIPK1 antibody (BA3852-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (BA3852-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa.
all(3)
Western blot analysis of RIPK1 using anti-RIPK1 antibody (BA3852-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse lung tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (BA3852-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa.
all(3)
ICC/IF analysis of RIPK1 using anti-RIPK1 antibody (BA3852-2).
RIPK1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-RIPK1 Antibody (BA3852-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)

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产品简介>

产品名称

Anti-RIPK1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Cell death protein RIP; Receptor interacting protein 1; RIP; RIP 1; RIP1; RIPK1; RIPK1-Specific

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

RIPK1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human RIP.

计算分子量

76 kDa

实际分子量

76 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RIPK1(Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al.(1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz(2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al.(1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.

Uniprot ID

Q13546

文献引用格式

RIPK1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3852-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

receptor(TNFRSF)-interacting serine-threonine kinase 1

蛋白名全称

Receptor-interacting serine/threonine-protein kinase 1

实验方案>

实验步骤

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