Anti-RIPK1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0169

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RIPK1 using anti-RIPK1 antibody (PB0169).
Lane 1: recombinant human RIP protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (PB0169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RIPK1 at approximately 38 kDa.
all(2)
Western blot analysis of RIPK1 using anti-RIPK1 antibody (PB0169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: JURKAT whole cell lysates,
Lane 2: 22RV1 whole cell lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: HELA whole cell lysates,
Lane 5: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (PB0169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa.
all(2)

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产品简介>

产品名称

Anti-RIPK1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell death protein RIP; Receptor interacting protein 1; RIP; RIP 1; RIP1; RIPK1; RIPK1-Specific

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

RIPK1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP.

计算分子量

76 kDa

实际分子量

76 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product(s) of RIPk1 upon stress.

Uniprot ID

Q13546

文献引用格式

RIPK1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0169)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

receptor (TNFRSF)-interacting serine-threonine kinase 1

蛋白名全称

Receptor-interacting serine/threonine-protein kinase 1

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实验步骤

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