Anti-RIPK1 Antibody (Clone#OTI2F1)

Mouse monoclonal antibody

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筛选器： All WB

M00141-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RIPK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK1.
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产品简介>

产品名称

Anti-RIPK1 Antibody (Clone#OTI2F1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell death protein RIP; Receptor interacting protein 1; RIP; RIP 1; RIP1; RIPK1; RIPK1-Specific

产品类型

Monoclonal

检验物种

human

应用范围

基因名称

RIPK1

克隆号

OTI2F1

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 133-422 of human RIPK1(NP_003795) produced in E.coli.

计算分子量

76 kDa

实际分子量

76 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product(s) of RIPk1 upon stress.

Uniprot ID

Q13546

文献引用格式

RIPK1 Antibody (Clone#OTI2F1) (Boster Biological Technology, Wuhan, China. Catalog#M00141-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

receptor(TNFRSF)-interacting serine-threonine kinase 1

蛋白名全称

Receptor-interacting serine/threonine-protein kinase 1

实验方案>

实验步骤

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