Anti-GFAP Antibody

筛选器： All WB IHC IF

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  Western blot analysis of anti- GFAP antibody (BM0055). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: U251 whole cell lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse brain tissue lysates.
  Use mouse anti- GFAP 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for GFAP at approximately 50KD. The expected band size for GFAP is at 54KD.

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  IHC analysis using anti- GFAP antibody (BM0055). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  IF analysis of GFAP, MBP using anti- GFAP antibody（BM0055）, anti- MBP antibody（BA0094）,detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP, DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126), rabbit anti-MBP Antibody , Cy3 Conjugated goat anti-rabbit IgG (BA1032) overnight at 4°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Western blot analysis of anti- GFAP antibody (BM0055). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: U251 whole cell lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse brain tissue lysates.
  Use mouse anti- GFAP 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for GFAP at approximately 50KD. The expected band size for GFAP is at 54KD.

• 

  IHC analysis using anti- GFAP antibody (BM0055). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

• 

  IF analysis of GFAP, MBP using anti- GFAP antibody（BM0055）, anti- MBP antibody（BA0094）,detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP, DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126), rabbit anti-MBP Antibody , Cy3 Conjugated goat anti-rabbit IgG (BA1032) overnight at 4°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.