Western blot analysis of GFAP using anti-GFAP antibody (M00213-8). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (M00213-8) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Histone H3, GFAP using anti- GFAP antibody（M00213-8）, anti- Histone antibody（A12477-2）,detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL mouse anti-GFAP, DyLight488 Conjugated goat anti-rabbit IgG (BA1127), rabbit anti-MBP Antibody , Cy3 Conjugated goat anti-mouse IgG (BA1031) overnight at 4°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(4) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of GFAP using anti-GFAP antibody (M00213-8). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (M00213-8) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Histone H3, GFAP using anti- GFAP antibody（M00213-8）, anti- Histone antibody（A12477-2）,detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL mouse anti-GFAP, DyLight488 Conjugated goat anti-rabbit IgG (BA1127), rabbit anti-MBP Antibody , Cy3 Conjugated goat anti-mouse IgG (BA1031) overnight at 4°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of GFAP using anti-GFAP antibody (M00213-8). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (M00213-8) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GFAP using anti-GFAP antibody (M00213-8).
GFAP was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GFAP Antibody (M00213-8) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Histone H3, GFAP using anti- GFAP antibody（M00213-8）, anti- Histone antibody（A12477-2）,detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL mouse anti-GFAP, DyLight488 Conjugated goat anti-rabbit IgG (BA1127), rabbit anti-MBP Antibody , Cy3 Conjugated goat anti-mouse IgG (BA1031) overnight at 4°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
