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Anti-HSP60/HSPD1 Antibody (Clone#LK1)

Mouse monoclonal antibody

说明书

筛选器: All WB IHC

BM1511

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of anti- HSPD1 antibody (BM1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1:Human HELA whole cell lysates,
    Lane 2:U87 whole cell lysates,
    Lane 3: Rat brain tissue lysates,
    Lane 4: Neuro-2a whole cell lysates.
    Use mouse anti- HSPD1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HSPD1 at approximately 68KD. The expected band size for HSPD1 is at 61KD.

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产品名称
Anti-HSP60/HSPD1 Antibody (Clone#LK1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
HSP 60; HSP60; HSP65; HSPD1
产品类型
Monoclonal
检验物种
human, mouse, rat, chicken
应用范围
WB,IHC
基因名称
HSPD1
克隆号
LK1
抗体来源
Mouse
抗体亚型
IgG1
免疫原
Recombinant human heat shock protein 60(HSP60)
计算分子量
61 kDa
实际分子量
60 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
Uniprot ID
P10809  
文献引用格式
HSP60/HSPD1 Antibody (Clone#LK1) (Boster Biological Technology, Wuhan, China. Catalog#BM1511)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
heat shock 60kDa protein 1(chaperonin)
蛋白名全称
60 kDa heat shock protein, mitochondrial
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-HSP60/HSPD1 Antibody (Clone#LK1)

    筛选器: All WB IHC

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