Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti- HSPD1 antibody (BM1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1:Human HELA whole cell lysates,
Lane 2:U87 whole cell lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Neuro-2a whole cell lysates.
Use mouse anti- HSPD1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HSPD1 at approximately 68KD. The expected band size for HSPD1 is at 61KD.
Figure 1. Western blot analysis of anti- HSPD1 antibody (BM1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1:Human HELA whole cell lysates,
Lane 2:U87 whole cell lysates,
Lane 3: Rat brain tissue lysates,
Lane 4: Neuro-2a whole cell lysates.
Use mouse anti- HSPD1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HSPD1 at approximately 68KD. The expected band size for HSPD1 is at 61KD.