Anti-GAPDH Antibody

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF

BM3874

100μl ￥880 200μl ￥1680 1ml ￥6800
货期：现货

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Figure 1. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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Figure 2. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat RH-35 whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GAPDH Antibody

规格/价格

100μl/880 200μl/1680 1ml/6800

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey, chicken

应用范围

WB, IHC, ICC/IF

基因名称

GAPDH

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human GAPDH

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Uniprot ID

P04406

文献引用格式

GAPDH Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM3874)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

glyceraldehyde-3-phosphate dehydrogenase

蛋白名全称

Glyceraldehyde-3-phosphate dehydrogenase

实验方案>

实验步骤

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