Anti-GAPDH Antibody

Mouse Monoclonal Antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3876

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-GAPDH antibody (BM3876). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GAPDH antigen affinity purified monoclonal antibody (BM3876) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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IHC analysis of anti- GAPDH antibody (BM3876).detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GAPDH Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

GAPDH

宿主

Mouse

抗体亚型

IgG

免疫原

A synthesized peptide derived from human GAPDH Mouse Monoclonal Antibody

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA

纯化方式

Affinity-chromatography

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Uniprot ID

P04406

文献引用格式

GAPDH Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM3876)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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