Anti-GAPDH HRP conjugated Antibody (Clone#A-7)

Rabbit Monoclonal Antibody

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筛选器： All WB

BM3896

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Figure 1. Western blot analysis of anti-GAPDH HRP conjugated antibody (BM3896). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH HRP conjugated antigen affinity purified monoclonal antibody (BM3896) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH HRP conjugated at approximately 36 kDa. The expected band size for GAPDH HRP conjugated is at 36 kDa.
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产品简介>

产品名称

Anti-GAPDH HRP conjugated Antibody (Clone#A-7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Monoclonal

检验物种

human, mouse, rat, chicken, fish, monkey, zebrafish

应用范围

基因名称

GAPDH

克隆号

A-7

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human GAPDH(HRP conjugated)

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Uniprot ID

P04406

文献引用格式

GAPDH HRP conjugated Antibody (Clone#A-7) (Boster Biological Technology, Wuhan, China. Catalog#BM3896)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Glyceraldehyde-3-phosphate dehydrogenase

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实验步骤

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