Anti-IkB Alpha/NFKBIA Antibody (Clone#HO-14)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF

BM3932

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-IkB Alpha/NFKBIA antibody (BM3932). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IkB Alpha/NFKBIA antigen affinity purified monoclonal antibody (BM3932) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IkB Alpha/NFKBIA at approximately 36, 39 kDa. The expected band size for IkB Alpha/NFKBIA is at 36 kDa.
all(3)
all(3)
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IkB Alpha/NFKBIA Antibody (Clone#HO-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

I kappa B alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

NFKBIA

克隆号

HO-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human IKB alpha

计算分子量

36,39 kDa

实际分子量

39 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals(NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Uniprot ID

P25963

文献引用格式

IkB Alpha/NFKBIA Antibody (Clone#HO-14) (Boster Biological Technology, Wuhan, China. Catalog#BM3932)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

NF-kappa-B inhibitor alpha

实验方案>

实验步骤

引用文献(0)>

清除