Anti-IkB Alpha/NFKBIA (Phospho-S32) Antibody (Clone#EDG-14)

Rabbit Monoclonal antibody

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筛选器： All WB IP

BM4411

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P-NFKBIA antibody (BM4411). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-NFKBIA antigen affinity purified monoclonal antibody (BM4411) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-NFKBIA at approximately 39 kDa. The expected band size for P-NFKBIA is at 36 kDa.
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产品名称

Anti-IkB Alpha/NFKBIA (Phospho-S32) Antibody (Clone#EDG-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

I kappa B alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA

产品类型

Monoclonal

检验物种

human

应用范围

WB, IP

基因名称

NFKBIA

克隆号

EDG-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-IKB alpha (S32)

计算分子量

36 kDa

实际分子量

39 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals(NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Uniprot ID

P25963

文献引用格式

IkB Alpha/NFKBIA (Phospho-S32) Antibody (Clone#EDG-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4411)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

NF-kappa-B inhibitor alpha

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实验步骤

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