Anti-IkB Alpha/NFKBIA Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB9291

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IkB Alpha/NFKBIA using anti-IkB Alpha/NFKBIA antibody (PB9291). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IkB Alpha/NFKBIA antigA03957-Aen affinity purified polyclonal antibody (PB9291) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IkB Alpha/NFKBIA at approximately 39 kDa. The expected band size for IkB Alpha/NFKBIA is at 36 kDa.
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产品名称

Anti-IkB Alpha/NFKBIA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

I kappa B alpha; IkappaBalpha; IkB alpha; IKBA; Ikβ-α; MAD 3; MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

NFKBIA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.

计算分子量

36 kDa

实际分子量

39 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals(NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Uniprot ID

P25963

文献引用格式

IkB Alpha/NFKBIA Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9291)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

蛋白名全称

NF-kappa-B inhibitor alpha

实验方案>

实验步骤

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