Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:50 |
Western blot analysis of anti-STAT3 antibody (BM4052). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (BM4052) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using STAT3 Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis of HeLa cells, using STAT3 Antibody.
Western blot analysis of anti-STAT3 antibody (BM4052). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (BM4052) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using STAT3 Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis of HeLa cells, using STAT3 Antibody.